Welcome to HepaPredict AB

Physiological hepatic in vitro systems for improved prediction of drug response and safety

We are a specialized CRO, focussing on drug metabolism, toxicity, and drug development for liver disease utilizing our advanced 3D primary human liver culture platform.

Our Services

Service philosophy: Delivering the best science by keeping things:

“As simple as possible and as complex as necessary.”

  • HepaPredict uses the most extensively characterized 3D spheroid system available, offering unmatched accuracy in predicting hepatotoxicity and evaluating toxicity mechanisms. We offer:

    • Tailored experimental designs and readouts.

    • Rapid turnaround of results and reports.

    • Extensive customer support from project initiation to closure.

    • Integrative analysis of results by experienced toxicologists.

    • Consulting about future steps and recommendations for follow-up studies.

    • Short-term exposures - Quick toxicity screenings to identify the most toxic drugs.

    • Long-term exposures - Repeated dose toxicity screenings for best DILI prediction.

    • Hepatocytes of different species: human, mouse, rat, minipig, dog, or monkey.

    • Compare DILI predictions in monocultures vs. cocultures with various NPCs.

    • Metabolic profiling

    • Pharmacokinetic analyses

    • Drug clearance assessments

    • Identification of enzymes responsible for drug metabolism

    • Short-term and long-term induction and inhibition studies of drug metabolizing enzymes, nuclear receptors, and other targets.

    • Various endpoints including viability, gene expression and immunofluorescence.

    • Modulation of gene expression

    • Knockdown or chemical inhibition of drug targets.

    • Overexpression of drug targets or other genes of interest.

    • Extensively characterized models for MASLD/MASH (NAFLD/NASH), fibrosis, and cholestasis.

    • Disease models are optimized to support the co-culture of hepatocytes with other primary non-parenchymal cells funtioning as a unique tool for disease mitigating drug screening.

    • Identification of compounds with disease inducing liability.

  • We offer various in-house assessments and endpoints which can be customized and optimized dependent on project needs, including:

    • Liver disease and co-culture models

    • Viability / Apoptosis

    • Gene and Protein expression

    • IHC / IF / Histology

    • Electron Microscopy

    • Omics Profiling

    • LC-MS

    • BSL-3 Facilities

    • AI Drug Repurposing

Advantages of the HepaPredict System

Our 3D spheroid platform offers multiple advantages over alternative in vitro systems and animal models:

  • Physiologically replicative in vitro system with close resemblance to human liver on transcriptomic, proteomic and metabolomic level.

  • Optimized to support co-cultures with non-parenchymal cells, such as Kupffer, biliary and Stellate cells.

  • Functionally stable and suitable for chronic applications, thus allowing to assess long-term toxicity, pharmacokinetics, and chronic drug response, not supported by conventional in vitro systems.

  • Easy manipulation of gene expression through knockdown or knockin techniques.

  • Emulation of human inter-individual variability in an in vitro setting.

  • High-throughput compatibility and scalability allows screening of hundreds of conditions and/or drug candidates with multiple replicates, adding to statistical power.

  • Controlled, chemically defined experimental conditions ensure maximal reproducibility.

  • Direct translation of findings to patients by using patient-derived cells instead of cell lines or animal models.

Let’s Work Together